Polat wakes up in Syria after being buried alive in a coffin, finding himself in a hotel with a note containing a name. As he tries to reach the person named and contact Istanbul, chaos unfolds there: Elif has had an accident, Safiye has been kidnapped, Nergiz's old health issues resurface, and Tuncay and İplikçi Nedim are detained by police while trying to flee Turkey. Kılıç falls into a trap while searching for Tilki Andrei. Nizamettin seems indifferent to these problems, while Halo's escape plan appears smooth. Polat, unaware of all this, is prevented from reaching Istanbul. Upon finding the person mentioned in the note, Polat discovers a bag left for him. As he tries to piece everything together, the Syrian Secret Service, Al-Mukhabarat, is on his trail.

Polat recalls his near-death experience in the coffin and how he was revived. In exchange for his new life, he's tasked with assassinating the Lebanese Prime Minister. While under constant surveillance, Polat investigates the motives behind this request, realizing that those using him aim to destabilize the Middle East and put Turkey in a difficult position. Those who revived Polat have already executed their plans for Nergiz, Doğu, and Esat. Elif fights for her life on the operating table, while Safiye wakes up in a room without doors, certain she'll be killed. Kılıç escapes the trap and disappears. As Polat's attempts to reach Istanbul are still thwarted, Nizamettin announces the first step of his war against the mafia and terrorism to the press.

Polat faces a sniper while trying to prevent an assassination attempt on the Lebanese Prime Minister, as Elif fights for her life on the operating table. Polat's efforts to reach Istanbul are still being thwarted. New allegations claim Polat has fled abroad after committing billion-dollar murders. Council member Tuncay is arrested, while İplikçi Nedim is released. Halo manages to hide without leaving a trace. Memati escapes the police and tries to meet with Erhan and Abdülhey. Nizamettin claims Polat no longer exists and advises everyone to stay away from his contacts. However, Nizamettin will be the first person Safiye sees while awaiting her death. Elif remains unconscious, fighting for her life. Polat's priority is to prevent the assassination of the Lebanese Prime Minister, unable to get news about his loved ones.

Polat, having defeated two snipers, realizes there are other plans for the Lebanese Prime Minister's assassination. While trying to dispose of a body, he faces a new problem. In Istanbul, Elif's loved ones are desperate as all doors close on them. Nizamettin cuts ties with everyone associated with Polat, including Elif. Memati, Abdülhey, and Erhan search for a hiding place. Tuncay finds himself in prison for trusting Abidin. Halo feels indebted to Polat. Laz Ziya learns to stay away from Nizamettin. Still unable to contact Istanbul, Polat devises a plan and briefly establishes contact. As the day of the assassination arrives, Polat's sole objective is to prevent the attack on the Prime Minister.

Polat Alemdar escapes from the hell of Aleppo and arrives in Istanbul with Nevzat, both filled with questions. He seeks out Halo, whom he had specially hidden, but others also know Halo's location. While saving Halo's life, Polat realizes that all his loved ones are in even greater danger. He asks Halo about the status of Elif, Safiye, Memati, Abdülhey, Erhan, Nizamettin, Kılıç, Tuncay, and Laz Ziya. Unable to get news about Elif, who lies hopeless in the hospital, Polat decides to rescue his sister Safiye first. Coincidentally, the secret forces have also chosen Safiye as their first target among the remaining individuals.

Upon learning about Elif's condition, Polat changes all his plans to reach her. Safiye faces either her brother Efe Karahanlı or Polat Alemdar, whom she believes has come to kill her. In shock, Safiye can't believe she might become a fratricide if she pulls the trigger again. Memati, unable to accept that Polat and Abdülhey are KGT agents, feels betrayed and leaves the team. Elif's gangrenous leg is scheduled for amputation, though she wishes for death to end her suffering. Nizamettin contacts the "Global Council" and is instructed to acquire the "Black Box of Turkey" from Karahanlı's house and eliminate everyone. Kılıç finds his own way to hide, intending to stay hidden until he finds answers. For Polat, only one goal remains before the end: reaching Elif.

For Polat Alemdar, being located is more dangerous than being wounded. Every corner of the hospital is surrounded by the police. Those who want to save him have to do something in their own way. Hikmet cannot escape arrest in this effort. When Safiye sees the relic Halo told her about, she realizes that it is not something ordinary. When she learns that what she sees is a cryptex and the “Black Box” of all secrets, a conflict awaits her. Kılıç has found answers to the questions in his head one by one. Now that he knows where to start, he decides to get out of the prison where he is hiding. For Polat, it is time to share the truth and his plans with his loved ones. In fact, this is a fork in the road for everyone...

Polat reveals the truth about himself. Polat begins to tell all his loved ones the truth about himself. In fact, this is both a farewell and a parting of the ways. Safiye, Halo and Memati, who do not find Polat's explanations sufficient, still want to know something more. Two important things remain for Polat. The first is to find the password and key needed to open the cryptex, and the second is to determine Nizamettin's connections. Elif's condition is getting worse and worse. Her only wish is to die as soon as possible. Kılıç secretly returns to Istanbul and tries to reach Nizamettin. When this surprise meeting takes place, everyone who tries to put together the cryptex, cipher and key will learn what they have and how much they have...

Polat and Nizamettin face off. At the end of Kılıç's surprise meeting with Nizamettin, everyone fighting for the cryptex has realized what the enemy has. Polat has the code and Nizamettin has the key. While Memati regrets not being able to protect Abdülhey, Nesrin's mistake will cost Laz Ziya dearly. Polat's ability to take action depends on reaching Nizamettin's connections. Elif, who has to live connected to the devices, is shocked by who comes to her. While Nizamettin bargains for Abdülhey's life, he also learns where Polat and his men are hiding. Polat will comply with Nizamettin's request.

The big finale of Valley of the Wolves! Polat Alemdar, who was born Efe Yakup Karahanlı, grew up as Ali Candan, then changed his face and his whole life, confronts the world barons on his way to crush the mafia. Will Polat, who risks everything to protect those he loves, be able to do the same against Lisa and Amon? On the other side, his beloved Elif, whom he could not give up even after becoming Polat Alemdar, is dying. Will Polat be able to see his beloved one last time before she dies? As Operation Valley of the Wolves comes to an end, Polat is answering for his loved ones and his life in court. How will Polat appear in court? What awaits him in court?